Light Microscopy Center

Our imaging services

• Widefield microscopy

  Booking site categories Light Microscopy and Adv. Light Microscopy

• Confocal microscopy

  Confocal microscopy

• Light sheet microscopy

  Light sheet microscopy

• Super-resolution microscopy

  • Triple color STORM
  • Zeiss LSM880 with Airyscan module
  • Zeiss LSM900 with Airyscan module
  • Leica SP8 with Lightning deconvolution

• Multiphoton microscopy

  • Lavision TriMScope II
  • PRIME website

• Live cell imaging

  • Leica SP8
  • Zeiss Axio Observer
  • Zeiss LSM880
  • Okolab
  • Incucyte

• Fluorescence lifetime imaging

  • Leica SP8 with FALCON TCSPC FLIM extension
  • Lavision TriMScope II

• FCS and single molecule detection microscopy

  • Leica SP8 with FCS
  • Olympus TIRF microscope with EMCCD camera
  • STORM

• Spectroscopic microscopy

  • Leica SP8
  • PhenoImager HT a.k.a Vectra Polaris

• Automated microscopy

  • PhenoImager HT a.k.a Vectra Polaris
  • Zeiss high-content Sample Finder AI
  • Leica automated high-content microscope
  • Incucyte
  • Leica SP8
  • Zeiss LSM880
  • Zeiss LSM900
  • Zeiss Axio Observer
  • Okolab

• Correlative Light/Electron microscopy

  • Correlative Light/Electron microscopy

• Sample preparation equipment

  • Laser dissection
  • Miscellanteous equipment

• Data analysis

  • Analysis on booking site
  • RTC Microscopy on Github

RTC Microscopy seminars

Experts and users of the RTC Microscopy present seminars on various microscopy setups and their applications.

For each edition of the RTC Microscopy Seminars, we aim to include one seminar on electron microscopy, one on light microscopy, and one on image analysis. The seminars target a broad audience and besides presenting the results of a microscopy-based research project, the presenters typically also provide a basic overview of the possibilities and pitfalls of the used techniques. The next RTC Microscopy Seminars will be announced soon. The past events can be found below.

• 11 July 2023

  Program

  • Luco Rutten (Medical Biosciences): The power of transmission electron microscopy: from basic to advanced TEM at Radboudumc
  • Malou Zuidscherwoude (Medical Biosciences): Advanced light microscopy approaches to understand the interaction between the calcium channel TRPV5 and calmodulin
  • Gert-Jan Bakker (Medical Biosciences): QUAREP-LiMi, a community-driven initiative for improved reproducibility in microscopy related research

• 4 April 2023

  Program

  • Robin van der Meijden (Medical Biosciences): 3D correlative live-to-cryo imaging: combining Raman, light and electron microscopy on Biological Tissues
  • Mark Gorris (Medical Biosciences): Studying Immune Cells in the Tumor Microenvironment: Multiplex Immunohistochemistry and Multispectral Imaging
  • Sjoerd van Deventer (Medical Biosciences): Combining dSTORM and cluster analysis to investigate the plasma membrane nanoscale organization

• 24 November 2022

  Program

  • Julie Verhoef (Medical Microbiology): (Cryo-)Expansion microscopy to visualize malaria parasites
  • Marcos Eufrásio Cruz (Biochemistry): Understanding the functionality of extracellular matrix vehicles using cryogenic transmission electron microscopy
  • Andrea Frielink-Loing (RTC Data Stewardship): Digital Research Environment for data storage, analysis, and collaboration

• 8 September 2022

  Program

  • Moritz Negwer (Human Genetics/Donders Center): Tissue clearing and light-sheet microscopy: whole-organ imaging at cellular resolution
  • Mariska Kea-te Lindert (Electron Microscopy Center, RTC Microscopy): 3D array tomography: an accessible method for 3D imaging of millimeter volumes at nanoscale resolution
  • Merijn van Erp (RTC Microscopy): Deep learning for the selective segmentation of cellular structures