Radboudumc Technology Center Stem cells

Stem cells Expertise and services

The Radboudumc Stem Cell Technology Center assists investigators in performing innovative research, ranging from molecules to man. It connects a broad range of research groups that have expertise in different stem cell fields with a focus on human induced Pluripotent Stem Cell (iPSC) technology. This provides a state-of-the-art service to reprogram different cell types into iPSCs that enable the development of an unlimited source of any type of human cell needed for study or therapeutic purposes.

Aims

• To generate experimentally validated human iPSCs: reprogrammed iPSCs are analysed for their stemness by quantitative reverse transcription and immunocytochemistry, and the characterisation can be extended to genomic integrity and differentiation capacities into the three germ layers.
• iPSC culture training and consultancy
• Consultancy of iPSC differentiation methods
• Consultancy on other stem cell cultures: human haematopoietic stem cells, human epidermal stem cells, human organoids, mouse embryonic stem cells (serum and 2i conditions)
• Joint expertise in different research fields: cell biology, genomics and epigenomics.
• Research projects based on collaboration

Areas of expertise

• Different stem cell fields with a focus on human iPSC technology
  • Dermatology
  • Epigenetics
  • Genetics
  • Haematology
  • Neurobiology
  • Tissue regeneration