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Electron Microscopy Center
The Electron Microscopy (EM) Center was founded in 2019 by Dr Anat Akiva and prof Nico Sommerdijk to support users in the application of electron microscopy, both using standard techniques as well as the most advanced imaging and analysis strategies.
read moreElectron Microscopy Center
The Electron Microscopy (EM) Center was founded in 2019 by Dr Anat Akiva and prof Nico Sommerdijk to support users in the application of electron microscopy, both using standard techniques as well as the most advanced imaging and analysis strategies.
Our special focus areas are 3D volume imaging, correlative light and electron microscopy, and cryo-electron microscopy, in which we work to push the techniques and methodology beyond the state-of-the-art . Through a recently awarded NWO-Groot project we now also serve as a National Facility for liquid phase EM of biological materials (BIOMATEM).
Interested? Please contact us!
Rona Roverts BAS
representative EM Center
contact form
Our imaging services
For contact regarding these services, please send an email.
- Scanning electron microscopy (+ EDX elemental mapping)
- Cryogenic scanning electron microscopy (vitrified samples)
- Room temperature transmission electron microscopy (+ EDX elemental mapping)
- Cryogenic transmission electron microscopy (vitrified samples)
- Liquid Phase Transmission Electron Microscopy
- 3D array tomography (serial sectioning) (embedded samples)
- 3D FIB/SEM volume imaging (embedded samples)
- Cryogenic 3D FIB/SEM volume imaging (vitrified samples)
- Room temperature correlative electron microscopy (embedded samples)
- (Fluorescence + TEM or 3D FIB/SEM)
- Cryogenic correlative electron microscopy (vitrified samples)
- FIB lamella preparation and lift-out for (cryo)TEM
- Raman microspectroscopy (2D/3D)
News, events and publications
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New state-of-the-art transmission electron microscope (TEM) at Radboudumcinstalled and in operation10 July 2023
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Large AI project receives over €95 million for ten years of public-private research23 September 2021
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New start-up Patholyt will bring AI research into pathology diagnostics21 September 2021
RTC Microscopy seminars
Experts and users of the RTC Microscopy present seminars on various microscopy setups and their applications.
read moreRTC Microscopy seminars
Experts and users of the RTC Microscopy present seminars on various microscopy setups and their applications.
For each edition of the RTC Microscopy Seminars, we aim to include one seminar on electron microscopy, one on light microscopy, and one on image analysis. The seminars target a broad audience and besides presenting the results of a microscopy-based research project, the presenters typically also provide a basic overview of the possibilities and pitfalls of the used techniques. The next RTC Microscopy Seminars will be announced soon. The past events can be found below.
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Program
- Luco Rutten (Medical Biosciences): The power of transmission electron microscopy: from basic to advanced TEM at Radboudumc
- Malou Zuidscherwoude (Medical Biosciences): Advanced light microscopy approaches to understand the interaction between the calcium channel TRPV5 and calmodulin
- Gert-Jan Bakker (Medical Biosciences): QUAREP-LiMi, a community-driven initiative for improved reproducibility in microscopy related research
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Program
- Robin van der Meijden (Medical Biosciences): 3D correlative live-to-cryo imaging: combining Raman, light and electron microscopy on Biological Tissues
- Mark Gorris (Medical Biosciences): Studying Immune Cells in the Tumor Microenvironment: Multiplex Immunohistochemistry and Multispectral Imaging
- Sjoerd van Deventer (Medical Biosciences): Combining dSTORM and cluster analysis to investigate the plasma membrane nanoscale organization
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Program
- Julie Verhoef (Medical Microbiology): (Cryo-)Expansion microscopy to visualize malaria parasites
- Marcos Eufrásio Cruz (Biochemistry): Understanding the functionality of extracellular matrix vehicles using cryogenic transmission electron microscopy
- Andrea Frielink-Loing (RTC Data Stewardship): Digital Research Environment for data storage, analysis, and collaboration
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Program
- Moritz Negwer (Human Genetics/Donders Center): Tissue clearing and light-sheet microscopy: whole-organ imaging at cellular resolution
- Mariska Kea-te Lindert (Electron Microscopy Center, RTC Microscopy): 3D array tomography: an accessible method for 3D imaging of millimeter volumes at nanoscale resolution
- Merijn van Erp (RTC Microscopy): Deep learning for the selective segmentation of cellular structures
Examples figures from our papers
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Correlation of FIB/SEM with light microscopy in 3D showing mitochondria (in purple) within cells of a zebrafish scale, under cryo conditions.
Link to the paper: www.nature.com/articles/s42003-023-04887-y
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A cryoFIB/SEM image and subsequent 3D reconstruction of a bacteria reveal both intracellular and protruding out vesicles.
Link to the paper: www.nature.com/articles/s41467-022-33054-w
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3D FIB-SEM segmentation showing viral particles (orange) in a vacuole (magenta) in close proximity to the nucleus (blue) of an infected cell in a kidney organoid.
Link to the paper: https://doi.org/10.1016/j.stem.2021.12.010
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3D volume reconstruction of a kidney organoid, imaged by FIB-SEM, showing podocytes (yellow, magenta) and foot processes.
Link to the paper: https://doi.org/10.1242/dev.200198
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3D reconstruction of cells (green) and their extracellular matrix (magenta) in a 3D cell culture system imaged with FIB/SEM. Link to the paper: https://www.sciencedirect.com/science/article/pii/S1047847724000364.
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