Facility Flow cytometry equipment

About our equipment

The technology center Flow cytometry holds nine flow cytometers (ranging from 4-colors up to 10-colors) and a flow sorter (18-color) that are available for research purposes. These are located within a ML-I/II biohazard room allowing analysis and sorting of genetically modified cells. A dedicated room is available for sorting viral-transduced and transfected cells with the required ML-I/II biosafety permit.

The research flow cytometers are supervised by senior specialists who train, advise and supervise internal and external personnel within the facility.

Beckman Coulter Cytoflex Flow cytometry analyzer

The core facility offers a CytoFLEX  flowcytometer which is a revolutionary system presenting optimal excitation and emission, minimizing light loss and maximizing sensitivity. The fully activated instrument includes five fluorescent channels from the 488 nm (Blue) laser, three from the  638 nm (Red) laser and five from the 405 nm (Violet) laser.

The following colors can be measured:

1. 488 nm excitation; FITC, PE, ECD, PECY5.5, PECY7
2. 638 nm excitation; APC, Alexa700, APC-Cy7
3. 405 nm excitation; BV421, BV510, BV605, BV711 en BV786

Acquisition is possible  with tubes and 96 (deep) well plates. Volumetric sample injection allows absolute cell counting. The system operates with the flexible Cytexpert software.
For reservations click here

Example applications

• Immunophenotyping (direct, intracellular, tetramer stainings)
• Viability (apoptosis)
• FRET Analysis (CFP YFP)
• Nucleic Acid Analysis (Cell cycle analysis)
• Cell function analysis (Ca efflux)
• Combinatorial encoding MiHA-multimer assay (Qdots)

Beckman Coulter Gallios Flow cytometry analyzer

The 10 color Beckman Coulter Gallios is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed datacollection.The system has 3 solid state lasers:

1. 488nm excitation; FITC, PE, ECD, PC5, PC7
2. 405nm excitation; Pacific Blue/BV421 and Krome Orange/BV510
3. 642nm excitation; APC, APC-A700 and APC-A750

A selection from up to 62 parameters can be processed per analysis, at acquisition rates of 25,000 events-per-second. Acquistion software is Kaluza G (2014) and it functions with a carousel (32 tubes).
For reservations click here.
 

Examples application

• Immunophenotyping (direct, intracellular, tetramer stainings)
• Viability (apoptosis)
• FRET Analysis (CFP YFP)
• Nucleic Acid Analysis (cell cycle analysis)
• Cell function analysis (ca efflux)
• Combinatorial encoding MiHA-multimer assay

Becton Dickinson Aria Flow cytometry sorter

The high-speed sorting cytometer is equipped with 5 lasers, allowing excitation with UV (355nm), 405,  488, 561, 640 nm. There are 18 fluorescence detectors, allowing analysis of 18 colors and FSC and SSC. This provides a powerful and flexible analysis platform suitable for a wide range of research applications. Cell subsets identified using this powerful analysis capability can if required be sorted, using an electrostatic deflection mechanism.

Analysis/sort rates of over 15000 cells per second are possible, and sorted cells (4 populations at the same time) may be collected in tubes, onto slides, or into the wells of microplates. There is a sample collection temperature control option available.The instrument is fitted with a robotic arm allowing the programmed deposition of specified numbers of particular cell types, for example into each well of a 96 well plate. Sorting purity should be >99%.

The following colors can be measured

• 355nm excitation (3 colors); Brilliant UV395, BUV496/Hoechst, Indo-1, BUV737
• 488nm excitation (3 colors); FITC/GFP, PerCP, PerCP-Cy5.5
• 405nm excitation (5 colors); CFP/Pacific Blue/Brilliant Violet 421 , Pacific/ Krome Orange/BV510, Qdot 605/ BV605, Qdot 705/BV711 and Qdot 800/BV786
• 561nm excitation (4 colors); PE, ECD/mCherry, PECy5, and PECy7
• 640nm excitation (3 colors); APC, Alexa 700 and APC-A750