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Research
Radboudumc Technology Centers
Mass spectrometry
Bioanalysis of pharmaceuticals and toxicants
What do we offer?
Bioanalytics to support mechanistic and translational pharmacology and toxicology. read moreWhat do we offer?
Bioanalytical knowledge and expertise at the department of Pharmacology & Toxicology is centered around quantification of small molecules in biological matrices in order to better understand the mechanistic aspects of pharmacokinetics, pharmacodynamics and drug-induced toxicity. Hence, our bioanalytics group supports both experimental preclinical as well as translational clinical pharmacological and toxicological work. Our focus is on determining exogenous molecules in common in vitro and in vivo biological matrices. In order to support mechanistic absorption, distribution, metabolism, excretion, toxicity (ADME-Tox) and pharmacology studies, we also quantify several endogenous small molecules that reflect the mechanism of specific drug effects. To illustrate our expertise and applications, listed below you can find a selection of our assays.We understand that in research, bioanalytical flexibility is crucial. Therefore, setting up new custom-made analytical methods is a service we can offer.
Applications
The possible applications explained. read moreApplications
Quantification of exogenous small molecules in various matrices (selection):Amiloride (plasma, urine)
Diclofenac and metabolites (plasma, bile)
Dipyridamole (plasma, tissue samples)
Eplerenone (plasma)
Furosemide (plasma, urine)
Hydrochlorothiazide (plasma, urine)
Metformin (plasma, urine, cell culture)
Caffeine (plasma)
NNAL, i.e. 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (urine)
N-methyl-quinidine (cell culture, membrane vesicle preparations)
Renal toxicity / renal function
Uremic toxins (urine, plasma)
Creatinine (via LCMS and hence applicable to mouse urine, plasma)
Iohexol (plasma)
Cholestasis
Bile salts (cell culture, membrane vesicle preparations)
Purinergic pharmacology and signaling
Adenosine (urine, blood and cell culture)
Adenosine phosphates: AMP, ADP, ATP (urine and cell cultures)
Cyclic adenosine monophosphate: cAMP (urine and cell cultures)
Equipment
Two triple quadrupole mass spectrometers coupled to a UPLC can be used:The first LC-MSMS system consists of a UPLC (Accela®, Thermo Scientific, San Jose, CA, USA), a quaternary ultra high pressure pump, a vacuum degasser and an autosampler, coupled to a (TSQ Vantage®, Thermo Scientific, San Jose, CA, USA) triple quadrupole mass spectrometer.
The second LC-MSMS system consists of a UPLC (Acquity® I-Class, Waters, Milford, MA, USA), a binary ultra high pressure pump, a vacuum degasser and an autosampler, coupled to a (TQ-S micro®, Waters, Milford, MA, USA) triple quadrupole mass spectrometer.
