28 December 2021

Cervical cancer remains a public health problem worldwide. Although we know that Human papillomaviruses (HPV) are the causative agent of the disease, there are still unknowns about how the virus induces carcinogenesis in women. Recent approaches have focused on studying the cervicovaginal microbiome and its association with HPV infections. However, profiling the microbiome to investigate the disease requires sequencing techniques that discriminate between microbial species, which current methods generally cannot achieve with high confidence. 

Hoping to improve microbiome profiling to fully understand HPV-induced cervical lesions, the group led by Willem Melchers, theme Women's cancers, and consisting of  Karolina Andralojc, theme Infectious diseases and global health and Mariano Molina from the Department of Medical Microbiology, in collaboration with William Leenders from the Department of Biochemistry, the CMBI, and the Departments of Pathology and Obstetrics and Gynecology, developed the method circular probe-based RNA sequencing (CiRNAseq). The results were published in the Journal BMC Biology on 16 December 2021.

They described the design and validation of probes that bind regions of interest in the ribosomal genes of more than 300 microbial species that are highly relevant for the cervicovaginal environment. They conducted in vitro experiments and demonstrated that CiRNAseq could perform DNA and RNA profiling and identify microbial communities and genera with high sensitivity. Lastly, they profiled a cohort of cervical smears that were either HPV negative without lesions or HPV positive with high-grade lesions and confirmed the well-described microbiome change that occurs upon HPV infection.

The cervicovaginal microbiome is associated with HPV infection, persistence, and carcinogenesis. This study demonstrates the potential of CiRNAseq for high-resolution microbiome profiling, which is essential to decipher the microbiome's role in women's cervical health and disease. They are currently analyzing the relationship between the microbiome and HPV in cross-sectional and longitudinal studies, aiming to elucidate viral carcinogenesis in women. 

Go to publication

Related news items


RIMLS awards call for nominations

19 October 2021 RIMLS awards several prizes to stimulate and honor our (young) researchers. Upcoming awards are Supervisor of the Year, Best Master Thesis, Best Publication, Best Image and more. Send your nominations now before 24 November 2021. read more

RIMLS online award ceremony proudly presenting the winners

13 January 2021

In this special webinar of the RIMLS New Year Celebration, scientific director René Bindels reviewed 2020 and looked forward to 2021. But more importantly a number of researchers received prizes in the traditional RIMLS awards ceremony. 

read more

Best publication award of 2019 in dermatology awarded to Jos Smits and Tom Ederveen

30 September 2020

The Dutch Society for Experimental Dermatology (NVED) awarded the publication in Journal of Investigative Dermatology of Jos Smits and Tom Ederveen.

read more

RIMLS PhD retreat registration is open

23 January 2020 Yearly, RIMLS PhD candidates gather for the two-day PhD Retreat. Apart from the science, this event is highly valued for the opportunity to meet and get to know fellow PhD candidates during the social activities. Early bird registration and abstract submission deadline: 4 March 2020. read more

RIMLS awards call for nominations

26 November 2019 RIMLS awards several prizes to stimulate and honor our (young) researchers. Please find here an overview of the upcoming awards. Deadline 12 December 2019. read more

RIMLS 25 years jubilee festival The Future is Yesterday

3 October 2019 Together with more than 250 colleagues and alumni we celebrated 25 years of research into the molecular mechanisms of disease. During this exciting and informal event, we not only took a trip down memory lane, but also looked ahead to the next 25 years and beyond…... read more